ISBN: 9781071621516
Код товара 150979
Directed Evolution
ISBN: 9781071621516
Код товара 150979
21 661 ₽
Доставка под заказ
Если Вы закажете книгу до 17.01.2025, то мы привезём её ориентировочно 28.02.2025.
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Автор
Currin
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Издатель
Springer
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Тип обложки
Hardback
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Размеры
254 x 178
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Год издания
2022
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Вес (г)
751
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ISBN
9781071621516
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Язык
ENG
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Кол-во страниц
278
О чём книга?
This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.
Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach.- Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR).- Non-Degenerate Saturation Mutagen
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