ISBN: 9781493991693
Код товара 145817
CRISPR Gene Editing
ISBN: 9781493991693
Код товара 145817
23 757 ₽
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Если Вы закажете книгу до 15.04.2024, то мы привезём её ориентировочно 05.06.2024.
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Автор
Yonglun Luo
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Издатель
Springer
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Тип обложки
Hardcover
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Размеры
254 x 178 x 22
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Год издания
2019
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Вес (г)
928
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ISBN
9781493991693
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Язык
ENG
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Кол-во страниц
362
О чём книга?
This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
CRISPR-gRNA Design.- Tracking CRISPR’s Footprints.- Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER.- Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA.- Functional Evaluation of
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